FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_3_NxW_C2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_3_NxW_C2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7495231
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC3601.0682015E-712.63982415
CGCCCAT11800.012.4596291
CGGGCAT10800.011.6685421
GCCGGAT15350.011.4024831
CGCGCGA3257.774803E-510.768949522
CGGCAAT19100.010.4467051
CCGGGAT19150.010.0538351
CGGGGGT3501.6430608E-410.0016071
CGCGTAT5603.0015144E-810.0016061
CGGGGAT11600.09.9584971
CGGGAAT19750.09.7484021
GCCCGAT8403.6379788E-129.5848721
CGCCCTT8453.6379788E-129.5281571
GGGAGAT41550.09.5201821
GCCGGCT11800.09.4930511
GCGGGAT19100.09.3470521
ATCGGGC7151.54796E-99.3008286
GCGGAAT14450.09.2056311
GCCGGAC10450.09.0445161
GGGGCAT11650.09.0143241
Produced by FastQC (version 0.11.5)