FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_2_NxW_C1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_2_NxW_C1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6838087
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC2602.7692295E-816.15471315
ATCGGGC5804.110916E-1010.8620966
CGGGGAT10850.010.6463561
CGCGCGG2650.001871940410.56598836
GCGGGTT15500.010.3882621
GCGGGCG4106.9491416E-610.24445314
GCCGAAT9950.010.202151
CCCACAT16450.09.7883321
ACGCGCG3259.278961E-49.6921253
GTGTGCG5805.179936E-89.6549148
CGCCAAT15450.09.5156321
CCGGGAT15650.09.3940261
CCGACAT17150.09.3888081
GCGAAAT21200.09.2463211
CGCCCAT11450.09.1713351
CGGCAAT16050.09.1599061
GCGCGCG3450.00160129289.13025864
CGGGCAT8851.0913936E-119.0970661
GGCGAAT20300.08.794091
GCCGGAT15550.08.779121
Produced by FastQC (version 0.11.5)