FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_24_NxW_C8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_24_NxW_C8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6355091
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT15250.011.0187381
CGGGAAT16700.010.6908991
CCCGCAT6251.3278623E-1010.6422641
CCGGCAT10000.010.5022341
CGGGCAT8650.010.117761
GCCGGAT13300.010.0021291
CCCCGAT5602.998604E-810.0021291
CGGGGAT10550.09.9547251
CGCCCTT7402.7284841E-119.9345461
CGGGCGC3208.0396485E-49.84483715
GTCCGCT5057.9229903E-79.7050351
CGCCCAT10200.09.6098881
CGCGCAT4056.779372E-59.5081971
CCCGTAT6654.3801265E-99.47571
ATCGGGC5957.676135E-89.4117686
GCCGAAC7901.0004442E-109.3057781
GTCGAAT21850.08.9721611
GCCGAAT9455.456968E-128.890781
TACCCTA7101.3507815E-88.87331240
GCGGGAT13850.08.8466471
Produced by FastQC (version 0.11.5)