FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_23_NxW_C8-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_23_NxW_C8-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4852243
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT5500.013.3662431
CCGGGGT6704.3655746E-1110.44981
GCGGAAT7401.8189894E-1210.4074341
CGCGAAT6452.3646862E-1010.3120891
CGCGTAT3853.8907616E-510.0019511
GCCGGGG3904.483248E-59.8737221
CGCACAT5006.9161433E-79.8019121
CCGGCAT8001.2732926E-119.6268781
GCGGGAT9500.09.5808161
GCGCAAT4107.7531215E-59.39207651
GGGAGAT26150.09.1031131
CCCGACT7402.8649083E-98.9882391
CCCGTAT5502.5290374E-68.9108291
CGCCCAT7101.3460522E-88.8749711
CGGGAAT13050.08.8523021
GGGAAAT25450.08.8032881
CGGGTAT7201.7091224E-88.7517081
GCCGACT6908.004645E-88.624871
GGGCAAT10203.6379788E-128.5801051
GGAAAAT41300.08.476231
Produced by FastQC (version 0.11.5)