FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_22_NxW_C8-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_22_NxW_C8-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12547816
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT14000.011.5021231
GCGGGAT20750.011.4719961
GCCCGAT9900.010.9616191
CGGGGAT21300.010.8470721
GCCAGAT42000.010.1685431
CGCGCAT7251.8189894E-1110.1398021
CGCGGAT16800.010.0018451
CGCGGGG5401.8910578E-79.7240171
GCCGAAT17800.09.636611
CGCCCAT18800.09.3102291
CGCCCTT12450.09.2788211
CTACACG18600.09.03291712
CGGGTAT16700.09.0136391
CCCCGAT11200.08.7516151
CGGGGAG14700.08.573011
CCGCAAT26750.08.5062431
GGGGAAT29700.08.4864151
TCCCTAC21900.08.4701969
CCGGGAT30250.08.447841
CGGGAAT35250.08.4412751
Produced by FastQC (version 0.11.5)