FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_21_NxW_C7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_21_NxW_C7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13169110
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT17050.013.9615551
GCGCGCG3255.9792656E-611.84580664
GCGGGAT21350.010.1658361
CGGGAAT36350.09.9193241
GCCGAAT17650.09.9168691
CCGGGAT33750.09.6462481
CCCGCAT13600.09.523841
CGCCAAT28100.09.4679621
GCCGGGG10450.09.3797451
GCCGGAT31850.09.3424061
CGCCCCT6603.902096E-89.0168381
CGGGACT17600.08.9505381
CGCGGAT18750.08.7749751
GCGGGAC9202.5465852E-118.75163651
CGCGAAT16250.08.6169971
CCGGGGG7756.5720087E-98.582251
CCGGCAT20800.08.4150351
CGGGGAT24450.08.3042121
CCCGAAT24100.08.2795561
CCGCAAT25400.08.2692621
Produced by FastQC (version 0.11.5)