FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_20_NxW_C7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_20_NxW_C7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5152106
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT4102.237357E-1013.660351
CGGGGAG6750.011.9275081
CTACACG7350.010.95330312
CGGGCCT3601.8573677E-510.6958631
CGCGTAT3601.8573677E-510.6958631
GCGAAAT14900.010.3369421
GCCACAT16000.010.0638351
CCCTACA7705.456968E-129.99987110
CGCACAT5401.8914216E-79.7235131
CCCGACT5805.165748E-89.6564531
GCGGCCT3652.507558E-49.5903141
GCCGAAT7352.5283953E-109.5250731
CGCACCC3702.8784128E-49.4595213
GCCAGAT17100.09.4164541
CGGGAAT14250.09.3345721
CGCCCGT3000.00522236159.3345721
CGCGGGT4552.3872783E-59.2319941
GCGGGAT8353.092282E-119.2227811
ATCGGGC4602.7203887E-59.1303176
CGGGTAT7302.244633E-99.1107981
Produced by FastQC (version 0.11.5)