FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_1_NxW_C1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_1_NxW_C1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9171465
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT10950.010.8691091
CGCGTAT6805.456968E-1210.8103451
ATCGGGC8950.010.5587066
TCGGGCG9350.010.4809887
CGGGCGC5451.9706931E-810.27656415
CCGGGAT24300.09.9396711
CGGGAAT26150.09.7719311
CGGACAT15750.09.55691
CCCCGCT6603.904279E-89.016421
CGGGGAT15950.08.9981321
CGGCAAT24050.08.8785891
CGCGGAT12700.08.8201391
CGCGCCT8758.185452E-118.8012391
GGCGGGC6806.335358E-88.75094513
CGCCAAT20450.08.7298351
CGCGGAG11500.08.5229391
CCGGCAT15700.08.472531
CTCGGAT40100.08.3802321
CCCACAT23850.08.3659571
CGGGGAG13400.08.35938551
Produced by FastQC (version 0.11.5)