FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_19_NxW_C7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_19_NxW_C7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9182873
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT5601.8189894E-1212.5023921
CCGGCAT15250.011.7071591
CGGGCAT10700.011.4507891
GCCGGAT22300.010.6746881
CCGGGAT24150.010.5817351
CGGGAAT23750.010.3177641
CGGGGGC10700.09.8131983
GCCGAAT11800.09.79000951
GCGGGAT15500.09.7115361
CGCGTAT6553.3778633E-99.6201611
CGCCCCT3803.75442E-49.2122891
GTCGGAT35500.09.1707691
CGGGGAT14600.09.1113321
GCGGGCT6151.2728196E-79.1074331
CGCGGAT13500.09.0758111
TCGGGGG10150.08.9657682
GTCGATT31100.08.8923771
CCCCACT10151.8189894E-128.6223391
GCCGTAT11900.08.5310441
CCGGGGT22450.08.420321
Produced by FastQC (version 0.11.5)