FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_18_NxW_C6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_18_NxW_C6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6082840
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT8350.012.5767981
CGGGGGT2902.4270883E-512.0708351
GGGGCCC2356.854846E-411.91468260
CCCGTAT5401.1823431E-1011.6684741
CCGGGAT14000.011.0017041
GCCAGAT18200.010.1938871
CCGGCAT10250.09.9039741
CGCCCAT9650.09.7942631
CGCGTAT3955.158643E-59.7483451
CGCCTAT6008.7105946E-89.334781
CGCCAAT13150.09.3170331
CGGGGAG8353.092282E-119.2229851
GCCGGAT13700.09.1985041
CCGGGTT19050.09.1877751
CCGCGTT6008.693278E-78.7513561
CGGGCAT6452.6398084E-78.6835161
CCCGTCT12250.08.5727571
GTCGAAT24150.08.5520491
CCGGCAC7001.0104668E-78.5013171
CCCGCAT6351.9612635E-68.2689971
Produced by FastQC (version 0.11.5)