FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_17_NxW_C6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_17_NxW_C6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9030490
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC5850.014.36058915
GCGGGCG5850.014.3604314
ATCGGGC7000.013.5001726
CGCGTAT6600.013.2601671
TCGGGCG7250.013.0340727
GGCGGGC6450.013.02435913
GGGCGCA7700.012.27431116
CGCCCCT6401.8189894E-1110.9396391
CGGGAAT23400.010.7713371
CGGCAAT21650.010.5101371
CGGGCAT9050.010.0572151
GTGTGCG6654.1654857E-109.9996848
CGCGCCT8450.09.9427721
CCCACAT18050.09.89112951
CGCGCCC3208.051235E-49.84338662
CGCCCTT8503.6379788E-129.472441
GCGCCCT4451.8396E-59.43811337
CGCCCAT16050.09.378781
GGCGCAT11300.09.29318317
CTCGCAT18550.09.247091