FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_16_NxW_C6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_16_NxW_C6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7494010
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCAT14500.010.3804731
CGCGTAT5251.2552118E-710.001121
ACCGCTA13250.09.77409349
GCGCGGG2950.00455354159.49119667
CGGGCAT6402.368688E-89.2979161
CCAGTCG14750.09.25385463
GGCGCGC3050.0059900679.18037638
AAAAAAC90350.09.10450651
GCCGAAT10550.08.9583491
GTCGAAT30100.08.9544911
CGCGGAT10250.08.8790441
GGCGCCC3550.0020770438.872931567
GAAAAAT68050.08.8474271
AGCGCGG4006.280128E-48.74969666
AGTCGGC14900.08.69097365
GGCGAAT25200.08.6120761
CCGGCAT12300.08.5375411
CGCGAAT10253.6379788E-128.5375411
CGCGGGG6603.7597601E-78.48455325
CAGTCGG15750.08.44409564
Produced by FastQC (version 0.11.5)