FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_15_NxW_C5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_15_NxW_C5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3829160
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAC2454.267353E-614.2885131
GTCGGAT14350.012.4414621
CGCCCAT5259.113137E-1011.3355541
CGCGGGT3752.3841567E-611.2021941
CGGGAAT10550.010.9500121
CCGGCAC5854.765752E-1010.771341
CGGGGAG4602.202105E-710.6542611
CGCGAGT4055.9907907E-610.3724021
CCGGGGT8850.010.2845011
CGGGCGT2850.00341760589.8264871
GCCGAAC3259.2587015E-49.6942061
CGAATCG9800.09.6432353
CGGGAGT6553.3742253E-99.6202051
CGGGCAA4804.352136E-69.4810241
GCCGATT7858.913048E-119.3648921
CCCCCAT3803.7529742E-49.2123311
CGGGGAT6503.0415322E-89.155641
CCCGAAT6503.0415322E-89.155641
ATTTAAC11100.09.1442636
CGCCTGT4602.7131282E-59.1322241
Produced by FastQC (version 0.11.5)