FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_14_NxW_C5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_14_NxW_C5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4943660
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC2155.6039426E-817.90675538
CGCGCAC2253.0371368E-513.999969540
GCCCGAT3951.873559E-913.2929941
GCGCGCA2405.570848E-513.12497239
CCCCGAT4406.87578E-1012.7290481
GTCCGCT4206.265327E-811.6682941
CGGGCAT5056.000846E-911.0906541
GCCAGAT16750.011.0761711
CGGGCCT3501.3596451E-511.00153351
CGGGAAT13400.010.971681
GCCGGGG3903.819121E-610.7707321
CCGGCAT8200.010.6722191
CGCCCCT3004.438063E-410.5014651
AGGGCCC4753.4355435E-710.31545421
CGCGATA3106.009014E-410.1616792
GCGGGCT3904.4860364E-59.8731711
CTACACG7151.4915713E-109.79098112
GCCGGAT11600.09.6565191
GCGCACC3652.511677E-49.5887341
TCCCTAC8051.4551915E-119.5650039
Produced by FastQC (version 0.11.5)