FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_13_NxW_C5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_13_NxW_C5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8888600
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT10500.013.0026571
CGGCAAT21600.011.669051
CGGGCGC4601.8037099E-811.4137715
GCGGAAT18850.011.3285741
GCGGGGC2650.001872459810.565762
CGGGGGT4401.431843E-610.3430221
GCCGGGG8250.010.1838991
CGCGAAT13150.010.1161361
CGGACAT15650.010.0659551
GCGGGAT18500.09.8398481
CGGGGAT15700.09.8109221
GCCGGAT21100.09.788731
GCGGGCG5452.1660708E-79.63358714
CGGGAAT23150.09.5267841
GGCGGGC5906.73881E-89.49180913
CCGGGAT20850.09.23451
CGCCCCT8353.092282E-119.2234411
CCGGCAT14450.09.2060331
GTCGAAT31350.09.04490951
ATCGGGC7909.931682E-108.8608756
Produced by FastQC (version 0.11.5)