FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_12_NxW_C4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_12_NxW_C4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9233451
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCCT4902.1827873E-1112.8597991
CGGGCAT10350.011.4999611
CGGGAAT22800.010.5942951
CGGGGCT8600.010.1765211
CGCCCAT13150.010.11615851
CGGGCGT5109.066025E-79.6098291
CGGCAAT21900.09.5910231
CGGGGAT15850.09.2763641
GCGGGAT14950.09.1323211
CGCGGAT11900.09.1195321
GCCGAAT13850.09.0993541
CCGGGAC13950.09.0341251
CCGGCAT15500.08.8082721
CCGGGAT21650.08.7315971
CGGACAT16900.08.4928791
GCCAGAT32250.08.46686651
CCCGGGT16300.08.3759641
GCGGGAC7553.833702E-88.3460961
CCCGTAT9403.5652192E-108.1931821
GGCAAAT37750.08.1606261
Produced by FastQC (version 0.11.5)