FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_11_NxW_C4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_11_NxW_C4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8930216
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT11600.013.5798751
GGCGCGC3106.0124235E-410.16123938
GCCAGAT29100.010.1047961
GCGCGCA4251.0664566E-59.88235939
CGGGGAT13600.09.781061
CGGGCAC10400.09.761261
GCCGAAT11300.09.6033891
CGCCCAT13000.09.4246651
CGCGGAT12550.09.2047391
CGCCCTT9900.09.1934691
CGCGCAT5007.316188E-69.1015341
CCGCGTT8555.0931703E-119.0073661
CCGGCAT14400.08.7514751
CGGGAAT24050.08.7332811
GCGGGAT14900.08.692741
GCCCGCT4451.8916257E-48.6531441
GCGGAAT18400.08.5612251
CGGGGCT9454.5474735E-118.5199551
GGAAAAT73450.08.483391
CGGGGAG11600.08.44971
Produced by FastQC (version 0.11.5)