FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_10_NxW_C4-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_10_NxW_C4-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7500764
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG2153.2397002E-413.02319136
CGGGCAT11700.012.8658431
CTACACG8750.012.40117912
CCGGGAT18500.011.7320971
CGCGAAT9600.011.3043631
GGCGCGC3551.5943891E-510.84465568
GCGGAAT15950.010.7545191
CGGGAAT20600.010.7060431
GCGGGAT16900.010.3571191
GCCGGAT18300.010.3299531
CCCTACA11300.010.22112110
CCGGCAT13750.010.1838721
CGGGGAT13800.010.1469751
CGGGCGC3501.6437075E-410.00121715
GCGGGCG3208.039668E-49.84488314
TCCCTAC11850.09.7465289
CGGGCAC8301.8189894E-129.7007521
GCGGGTT16650.09.6716191
GGGCGCC2950.0045534249.49122524
GCCGAAT10800.09.40004351
Produced by FastQC (version 0.11.5)