FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_G11-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_G11-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653012
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGATTGCCTGTGAGGCCAATGCCTGGGATGTGCAGGCAGCACCT13920.2131660673923297No Hit
GCTTAGGAATGATGAGTGTGATTCTCTTCAGGCCCTGAGCTGTGTCATCC12570.19249263413229772No Hit
TAATAATACAGGTTGTAAGTAGCAAAACCAGTATTTAAGCACCATCCATT11590.17748525295094117No Hit
TTTCTATACCTCAGCCTTCTTTTCCACAGTCTTTCTAGGTCTTGTCTAAT11550.17687270678027356No Hit
GTACTGACTCTGTGGCTTCTGCCTGATGCTCAGCATCGACTGATGTGGGC11540.17671957023760668No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA9080.1390479807415484No Hit
TGTTACTACTGATGCCCTGGGGAGAAGCTGTTAGCCTCTCCTGTAGGTTC8780.1344538844615413No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT8400.12863469584019896No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG7960.1218966879628552No Hit
ATCCTGAGCACTGTGGGGGAAGAACAGGTTCCGGGAGAGGTACTAGGATT7910.1211310052495207No Hit
TTACTATACATTGGTTTATTAGGACAAGGTTTCTCATCTTCAGCACTACT7510.11500554354284453No Hit
ATACAGGAGAGCTCCCTACAACAAAAAGTTCCTTGGTCCAAAATACCAGT7440.11393358774417622No Hit
GATGTGGGCAGCGTGGGGGGAACCATCCTGAGCACTGTGGGGGAAGAACA7420.1136273146588424No Hit
CTTTATATGAGCCCCTTGGAGCAGAACAAGACCATCATTGTGGGCAGAGG7140.1093394914641691No Hit
CTTCAGGCCCTGAGCTGTGTCATCCAGGCACCTTCCCAGAGCATTTAGGT6940.10627676061083105No Hit
GGAATGATGAGTGTGATTCTCTTCAGGCCCTGAGCTGTGTCATCCAGGCA6840.10474539518416201No Hit
TACATACACCGCACAGACAAGATCCTGAGGAGGTAGAGTCTGAGGACAGT6740.10321402975749297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)