FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_E08-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_E08-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741776
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA18810.2535805957593667No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC16990.2290448868661159No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC13930.18779254114449645No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA13570.1829393240007765No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT13500.18199564288949763No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA13430.18105196177821875No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG12340.16635749875973338No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC11900.16042578891740902No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC11470.15462889066241023No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGG10900.14694463018485365No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG10490.14141735510450595No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG10270.13845150018334376No Hit
CTTGTATATGGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGGGCTGGGAT10210.13764263065939045No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG10040.13535083367485604No Hit
CTCCTATTGTGACTGGGTGTACATTCTGGAAAGGAAGACTACTGTTTATA9850.1327894135156705No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC9560.12887987748322943No Hit
GTATATGGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGGGCTGGGATGTT9230.12443109510148617No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT9130.12308297922823062No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC8720.11755570414788291No Hit
ATCATAATGTGAGTCAAACCAGCCGTTGCATCATCACCATTGTTGGCTTG8450.11391579129009295No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA8450.11391579129009295No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA8050.10852332779707081No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA7880.1062315308125364No Hit
CTATTAATAGTGGCCGGATTTTTTCAATTTTCTTTCTTGTTGAATCATTG7720.10407454541532754No Hit
GTGTATGGACCTGCCGTAGCCAGTGGGTACGACTTCGAAAAAGAGGGATA7640.1029960527167231No Hit
GTATTAATGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGA7630.10286124112939755No Hit
ATCCAGAACAGCTTAACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGA7570.10205237160544423No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA7560.10191756001811869No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA7560.10191756001811869No Hit
ACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGAGAGGAGGAATAAATA7460.10056944414486314No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)