FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_D10-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_D10-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654508
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT18440.28173834391634633No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT18310.2797521191490402No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT16730.25561184890024263No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA15870.24247220813190976No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA15350.23452730906268526No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA15120.23101321908975903No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC14890.2274991291168328No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT14580.22276274697941048No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA14430.22047094917098034No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT13540.20687294884096144No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA13340.20381721842972125No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA13200.2016782071418531No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT12960.19801133064836487No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA12950.19785854412780285No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG12900.1970946115249928No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT12800.1955667463193727No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC12700.19403888111375261No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA12640.19312216199038057No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA11440.17478777952293936No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT11280.1723431951939472No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC10950.1673012400154009No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT10900.16653730741259082No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC10890.16638452089202882No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA10890.16638452089202882No Hit
GTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAAGAGTA10430.15935634094617637No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT10410.15905076790505235No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC10330.15782847574055625No Hit
TATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGAACAA10190.1556894644526881No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA9950.1520225879591999No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC9890.15110586883582783No Hit
GAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT9730.14866128450683566No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTG9710.14835571146571164No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG9660.1475917788629016No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA9350.14285539672547928No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG9330.14254982368435526No Hit
TCCCAGCACTGGGATGCTCTTCTAGGTATTTATTTCTTCTCTCATCAAAA9230.14102195847873517No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC9200.14056359891704914No Hit
ATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACC9120.13934130675255305No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG9100.13903573371142905No Hit
AATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTG8830.13491049765625476No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC8710.13307705940951067No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCTC8590.13124362116276655No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT8570.13093804812164253No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA8350.1275767446692783No Hit
GTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTGGGAAG8290.12666002554590625No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA8280.12650723902534422No Hit
ATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGA8200.12528494686084815No Hit
TCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAA8180.12497937381972413No Hit
TTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAA8060.12314593557298001No Hit
ATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGA7890.12054856472342584No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT7810.11932627255892977No Hit
CTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGC7780.11886791299724374No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT7780.11886791299724374No Hit
TTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTC7740.11825676691499569No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC7650.1168816882299376No Hit
CCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTT7590.11596496910656555No Hit
GATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAA7580.11581218258600354No Hit
ATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATAT7530.11504824998319348No Hit
TAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAA7400.11306202521588736No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT7180.10970072176352313No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA7160.10939514872239911No Hit
GTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTTCCTGGG7120.10878400264015109No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT7100.10847842959902707No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG6920.10572827222891089No Hit
AGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAACAGGC6910.10557548570834888No Hit
TCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGAGTTGGAACAA6740.10297811485879471No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA6730.1028253283382327No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC6660.10175582269429861No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT6660.10175582269429861No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG6620.10114467661205058No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)