FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_D09-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_D09-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402330
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA11480.2853379066935103No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC11440.2843436979593866No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC9560.23761588745557133No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA9060.22518827827902468No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA8150.2025700295777098No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG8000.19884174682474587No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT7260.18044888524345687No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC6770.16826982825044118No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC6680.1660328585986628No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT6670.16578430641513187No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC6390.15882484527626575No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGG6280.1560907712574255No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT5590.13894067059379117No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG5490.13645514875848186No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG5190.12899858325255387No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG5180.12875003106902294No Hit
CAATAGGAGAGTGCCCAAAGTATGTCAGGAGTACAAAATTAAGGATGGTT5110.1270101657843064No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA4960.12328188303134242No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA4940.12278477866428056No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC4920.1222876742972187No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA4880.12129346556309496No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4840.12029925682897123No Hit
CTCCTATTGTGACTGGGTGTACATTCTGGAAAGGAAGACTACTGTTTATA4830.1200507046454403No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC4770.11855939154425472No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC4660.11582531752541447No Hit
ACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGAGAGGAGGAATAAATA4600.11433400442422888No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA4460.11085427385479582No Hit
GTATTAATGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGA4440.11035716948773396No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA4420.10986006512067209No Hit
ATCCAGAACAGCTTAACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGA4420.10986006512067209No Hit
GTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGCATTTCCTGGG4410.10961151293714115No Hit
GGCTACGGCAGGTCCATACACACAGGCAGGCAGGCAGGACTTGTGAGCAA4240.1053861258171153No Hit
GTGTATGGACCTGCCGTAGCCAGTGGGTACGACTTCGAAAAAGAGGGATA4080.10140929088062038No Hit
ATCATAATGTGAGTCAAACCAGCCGTTGCATCATCACCATTGTTGGCTTG4050.1006636343300276No Hit
CTCCAGAAGTTTCTGTCATTGATTCCACGTTTGATCATCCTGATCAACTC4030.10016652996296574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)