FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_D08-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_D08-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740736
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA14180.19143122515984104No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC13320.17982115085536549No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA12930.17455611715914982No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT12570.16969608605495073No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC11310.152685977190254No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT11030.1489059529980992No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC10880.14688094003801624No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG10730.1448559270779333No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA10120.136620874373596No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA10120.136620874373596No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC9730.13135584067738032No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC9450.1275758164852255No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG9360.12636080870917574No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA9330.12595580611715915No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT9300.12555080352514258No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA9090.12271578538102644No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC8990.12136577674097114No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC8970.1210957750129601No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGG8770.1183957577328495No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC8730.11785575427682737No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT8700.1174507516848108No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC8530.11515573699671679No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG8450.11407573008467255No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG8180.11043070675652324No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG8040.10854069466044583No Hit
CTCCTATTGTGACTGGGTGTACATTCTGGAAAGGAAGACTACTGTTTATA7880.10638068083635736No Hit
CTTGTATATGGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGGGCTGGGAT7860.10611067910834629No Hit
GTCCATATTATTTGGATCCCCATTCCCATTAAGGGCATTTTGGACAAAGC7800.10530067392431311No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG7640.10314066010022463No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)