FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_C12-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_C12-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727480
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG16370.2250233683400231No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG15100.20756584373453568No Hit
CTATAGTACTGGTCATACCTATTTTACGAATAGAGAAATGAAGTTTACTT14720.20234233243525596No Hit
GTATGATACATGGGGAGGCTCCTTAGGGAGAGTAAGAATTAAATGAACAC9600.13196239071864518No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT9550.13127508660031892No Hit
GAGTAGAGAATGAGTAGGACTGAGCTAAATAAAAGGATGGCAGTGGGGTG8320.11436740528949249No Hit
TTCATAAAGTGATTCATTTGTTAGGAACATACAGCCATCCAGACGCAGTG8100.11134326716885688No Hit
CCTTAGGGAGAGTAAGAATTAAATGAACACACAGAGTAGAGAATGAGTAG7530.10350800021993731No Hit
CTATTAATATAGGGCAACATTTCCCAAAGCGTGGTATGCTGAACGCTGAC7500.10309561774894155No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)