FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_C11-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_C11-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628255
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG29540.4701912440012415No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG14360.22856960947386012No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT12500.1989637965475802No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG11970.1905277315739628No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC9890.15742015582844546No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA9860.15694264271673128No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA9130.14532315699835258No Hit
CTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGG7710.12272086971054748No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA7290.11603568614654877No Hit
ATCTTATACCATGAGCATTTTCCCACAATCATGTGCTATCCCTCCCTTAC6620.10537122665159847No Hit
TTTCAAATACTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACC6590.10489371353988428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)