FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_B01-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_B01-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789320
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA17660.2237368874474231No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT14110.1787614655652967No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC12040.15253636041149343No Hit
CTATTAATATAGGGCAACATTTCCCAAAGCGTGGTATGCTGAACGCTGAC11420.14468149799827698No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA11100.14062737546242335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)