FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_A12-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_A12-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559632
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG22190.39651056408497015No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG10770.1924478943305601No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG10520.18798067301369473No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC8590.15349372444749407No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT7760.13866254967550104No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC7310.13062155130514336No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA6530.11668382079652344No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA5740.10256740143522887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)