FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_A11-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_A11-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691760
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT15390.22247600323811725No Hit
CTATTAATAGTGGCCGGATTTTTTCAATTTTCTTTCTTGTTGAATCATTG14940.21597085694460508No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC12810.18517983115531397No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT11580.1673990979530473No Hit
GTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA10700.15467792297906788No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC9520.13761998380941368No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCATCAATGAGCA9080.13125939632242398No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA8760.12663351451370417No Hit
ATATAATGCAGAATTGTTGGTTCTACTGGAAAATGAAAGGACTTTGGATT8740.12634439690065918No Hit
ATTCAATAGTCTGTATGCATCTCCACAATTAGAAGGATTTTCAGCTGAAT8490.12273042673759686No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC8360.12085116225280443No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT7870.11376778073320228No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC7840.11333410431363479No Hit
GTCATAAGTTCCATTTTTCACACTTTCCATGCATTCATTGTTACACTTGT7810.11290042789406732No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA7490.10827454608534752No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA7400.10697351682664508No Hit
GTATATGTGAACTTCCCTCCTTGTCACTCCAATCTCGATGAATCTATTCT7390.10682895802012259No Hit
GGTTCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT7310.10567248756794263No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC7280.10523881114837515No Hit
GTTTGTATACTTTGTTGAGACACTGGCAAGAAGTATATGTGAAAAACTTG7270.10509425234185267No Hit
GAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAAATCCTTCTAATTGT7240.10466057592228518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)