FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_A08-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_A08-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789715
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA17080.21628055690977124No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC15790.1999455499768904No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC13360.1691749555219288No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA12570.15917134662504828No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT12260.15524587984272809No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA11920.15094052917824785No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC11840.1499275054924878No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC11190.14169668804568736No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC11170.14144343212424737No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG10990.13916412883128723No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGG10550.13359249855960695No Hit
CTCCTATTGTGACTGGGTGTACATTCTGGAAAGGAAGACTACTGTTTATA10190.1290338919736867No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC9720.1230823778198464No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG9490.12016993472328626No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC9450.11966342288040623No Hit
CTATTAATAGTGGCCGGATTTTTTCAATTTTCTTTCTTGTTGAATCATTG9340.11827051531248615No Hit
GTATATGGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGGGCTGGGATGTT9320.11801725939104614No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG9180.11624446794096603No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT9130.115611328137366No Hit
CTTGTATATGGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGGGCTGGGAT9070.11485156037304596No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG8760.11092609359072576No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA8670.10978644194424572No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT8480.10738051069056559No Hit
ATATAATGCAGAATTGTTGGTTCTACTGGAAAATGAAAGGACTTTGGATT8310.10522783535832547No Hit
GTCAAAAATCACACTGAAGTTCGCTTTCAGTATGATGTTCTTATCCATTA8270.10472132351544544No Hit
ATCATAATGTGAGTCAAACCAGCCGTTGCATCATCACCATTGTTGGCTTG8240.10434143963328542No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA8040.1018088804188853No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA7950.10066922877240522No Hit
GTCATAAGTTCCATTTTTCACACTTTCCATGCATTCATTGTTACACTTGT7920.10028934489024523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)