FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_A04-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_A04-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554998
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA7490.13495544128086948No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG7210.12991037805541641No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT6850.12342386819411962No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA6810.12270314487619777No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT6350.1144148267200963No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA6230.1122526567663307No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)