FastQCFastQC Report
Thu 27 May 2021
HTHHKBGXH_n01_S11_R1_A03-fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHKBGXH_n01_S11_R1_A03-fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586866
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG15070.25678775052567365No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT14930.25440219743518966No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA14170.24145205208684775No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13730.2339545995167551No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA12760.2174261245326872No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA12740.21708533123404658No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12570.21418858819560174No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT12320.20992867196259454No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA12260.20890629206667283No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA11590.1974897165622135No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11120.18948107404416No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT11000.18743631425231652No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10500.17891648178630215No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT9740.1659663364379603No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA8840.1506306379991344No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC8540.14551873851952576No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT8530.14534834187020546No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT8380.14279239213040112No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC8380.14279239213040112No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC8100.1380212859494331No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA8040.13699890605351137No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC7910.13478374961234763No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG7820.13325017976846504No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC7530.12830867693817669No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG7430.12660471044497382No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT7180.12234479421196663No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG7050.12012963777080288No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT6960.11859606792692028No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG6830.11638091148575655No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT6740.11484734164187395No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA6690.11399535839527251No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG6680.11382496174595223No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT6670.11365456509663194No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC6580.11212099525274935No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT6520.11109861535682762No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT6470.11024663211022619No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG6460.1100762354609059No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG6430.10956504551294503No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT6320.10769068237042187No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA6250.10649790582517986No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC6180.10530512927993785No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA6060.10326036948809438No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC5990.10206759294285236No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC5970.10172679964421179No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)