FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29711509
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1150810.38732802160940394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA236600.051.440922
GATCGGA237200.051.2799341
TCGGAAG236850.049.907983
CGGAAGA238350.048.8598754
ACACGTC185300.047.6659713
CACACGT201150.045.5081112
CGTCTGA184600.044.97924416
GCACACG206950.044.7056411
CACGTCT195500.044.2854814
ACGTCTG190250.044.2116215
AGAGCAC270100.038.1608628
AGCACAC266450.037.12221510
GAACTCC233450.033.0495321
CTGAACT252300.032.30410819
TGAACTC283300.028.993520
AACTCCA276500.028.09628522
GAAGAGC398900.027.6179876
GAGCACA381300.026.6283599
CTCCAGT263900.026.30507924
CCAGTCA229850.025.61159526
Produced by FastQC (version 0.11.5)