FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19078708
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT30150.040.40878742
TCGTATG30550.040.22001643
CGTATGC32000.038.6171444
TCTCGTA32350.037.553841
TATGCCG33550.037.4558646
ATGCCGT33800.036.5548647
ACGGCTA37500.033.22542632
ATCTCGT39500.031.28838340
TGCCGTC41800.029.56057548
CGGCTAC43350.028.8241933
CCGTCTT46050.026.8327550
GCCGTCT47250.026.07720249
CATCTCG47450.025.97191639
TCACGGC51850.024.63739630
CGTCTTC51650.023.92198451
GTCACGG53750.023.5057329
AGTCACG57100.022.61707128
CACGGCT59050.021.51480531
GTATGCC59550.020.92599345
CGTCTGA70850.018.42194416
Produced by FastQC (version 0.11.5)