FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG078.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG078.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21054110
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT21150.027.31317142
TCGTATG22550.026.08093343
CGTATGC23400.025.13354944
TCTCGTA24350.023.86901741
TATGCCG25250.023.15107246
ATGCCGT27250.021.8360347
TATCTCG26800.021.686639
TGCCGTC33150.018.37267748
ATCTCGT33450.017.68993640
CCGTCTT37400.016.1920950
GCCGTCT38250.015.83226649
CGTCTTC44200.013.77950851
GTATGCC52800.011.93324945
GCTTATC56400.010.67714436
CGTCTGA60700.010.433660516
ACGTCTG66500.09.418467515
TAGCTTA73900.08.71564934
ACACGTC70750.08.70430513
AGCTTAT76450.08.37986635
GTCACTA80300.08.3682729
Produced by FastQC (version 0.11.5)