FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG063.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG063.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23525186
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC9365663.9811204893342818TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1030150.065.6231842
TCTCGTA1028900.065.5254941
TCGTATG1046050.065.5171143
CGTATGC1049250.065.35944
ATGCCGT1050250.065.2337347
TATGCCG1051250.065.2055246
CGATCAG1051150.065.12057533
ACGATCA1056600.065.0321532
TGCCGTC1054500.064.9435648
GCCGTCT1054800.064.9264749
CCGTCTT1056750.064.8163250
GATCTCG1038900.064.707939
CGTCTTC1059900.064.6424151
GTCACGA1063250.064.585529
ATCTCGT1042050.064.5448740
CACGATC1064450.064.5424231
TCACGAT1065600.064.4634630
AGTCACG1076750.063.8056728
GTATGCC1076200.063.7102345
CGTCTGA1121850.062.0872616
Produced by FastQC (version 0.11.5)