FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19387949
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2483201.2807956117483081TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG282700.063.656639
TCTCGTA282950.063.539841
CTCGTAT283550.063.4506442
TCGTATG287400.063.447843
ATGCCGT289750.062.8616147
CGTATGC291000.062.84332744
TATGCCG291300.062.6149546
TGCCGTC292050.062.31064648
GCCGTCT293000.061.88419349
CCGTCTT296300.061.49046350
CGTCTTC297400.060.99043751
ATCTCGT295500.060.94818540
CGTCTGA321150.059.94813516
ACGTCTG326400.059.03764715
GTATGCC313700.058.41211745
ACACGTC333650.058.01638813
CACGTCT341250.056.67273714
ATCGGAA364850.053.625412
CACACGT362800.053.53831512
TTGAATC340550.053.34535236
Produced by FastQC (version 0.11.5)