FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20056631
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1224110.6103268290671549TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG148500.062.02160343
CTCGTAT147450.061.89748842
TCTCGTA148050.061.3430641
CGTATGC151150.061.0742544
TATGCCG152300.060.6310846
ATGCCGT155050.059.59747
ATCTCGT157500.057.75196540
TGCCGTC163300.056.6948648
CATCTCG163350.055.65991639
CCGTCTT166450.055.53906250
GCCGTCT169100.054.68939649
CGTCTGA174950.053.58373616
CGTCTTC174450.053.0494151
GTATGCC174800.052.94715545
ACACGTC178250.052.6112313
ACGTCTG179600.052.21615215
CACGTCT192100.048.90902314
ATCGGAA196300.048.5600852
GATCGGA196400.048.4399261
TAGGCAT203300.045.37195635
Produced by FastQC (version 0.11.5)