FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25781547
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1787240.6932244989022576TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT211100.062.42505342
TCGTATG217000.061.9812943
TCTCGTA212150.061.6731541
CGTATGC219400.061.3363844
ATGCCGT221750.060.70651647
TATGCCG222700.060.4527146
ACGATCT220400.059.12310837
CACGATC220700.059.010936
CGATCTC221400.058.9203438
TCACGAT227000.058.43279335
GATCTCG224400.058.2887739
ATCACGA229700.058.1530434
ATCTCGT225400.058.0019240
TGCCGTC232850.057.89025548
CCGTCTT235650.057.2489650
GCCGTCT237600.056.73470349
CATCACG239000.056.1471733
CGTCTTC243750.055.3006651
GTATGCC251550.053.6593445
CGTCTGA266150.051.54949616
Produced by FastQC (version 0.11.5)