FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20947038
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1907330.910548784988121TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG215300.062.6499143
CTCGTAT212650.062.6119942
TCTCGTA213500.062.15221441
CGTATGC217200.062.13261444
TATGCCG218850.061.6089846
AATCTCG215950.061.49498439
ATGCCGT222200.060.7710247
ATCTCGT223550.059.35864340
CGTCTGA237600.058.99572416
TGCCGTC229650.058.7409948
ACACGTC241000.058.2358713
CCGTCTT232400.058.1389150
ACGTCTG242700.057.8283715
GCCGTCT237100.056.97166449
ATCGGAA251450.056.3075332
GATCGGA252500.056.1597141
CGTCTTC240750.056.1205851
GTATGCC242700.055.6704745
CACGTCT254650.055.05955514
GTAATCT248550.053.40006337
Produced by FastQC (version 0.11.5)