FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19619710
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1505430.7673049193897361TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT178350.062.17047542
TATGCCG181350.061.630346
TCGTATG181450.061.52669543
TCTCGTA181150.061.0765641
CGTATGC183400.061.02506644
ATGCCGT184200.060.55808347
ATCTCGT189550.058.55643540
TGCCGTC195850.057.03022448
CCGTCTT198100.056.52628350
GCCGTCT197800.056.4176449
CATCTCG197850.055.9916139
CGTCTGA205750.055.76400416
ACACGTC209600.054.9735713
CGTCTTC203700.054.8835751
ACGTCTG209600.054.78993615
GTATGCC205450.054.48742745
GATCGGA219600.053.01321
ATCGGAA220650.052.68312
CACGTCT218650.052.6500514
GATCATC218950.050.88216436
Produced by FastQC (version 0.11.5)