FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20413508
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC2035290.9970309855611293TruSeq Adapter, Index 14 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG235100.063.46233743
CTCGTAT231950.063.4248642
CGTATGC236600.063.10407344
TCTCGTA232350.062.7912141
TATGCCG240100.062.22213746
ATGCCGT241150.062.00547847
ATCTCGT241450.060.39656440
TGCCGTC252050.059.32663748
CGTCTGA259050.058.797416
ACACGTC261250.058.58310713
GCCGTCT256100.058.41792349
CCGTCTT256300.058.33151250
ACGTCTG263450.057.85539215
CATCTCG253350.057.57281539
ATCGGAA271650.057.271332
GATCGGA271800.057.229681
GTATGCC262950.056.86864545
CGTCTTC264200.056.66527651
CACGTCT273300.055.8338314
TCGGAAG289850.053.685173
Produced by FastQC (version 0.11.5)