FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20871171
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2823211.3526840444170574TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG331150.063.8398843
CTCGTAT326550.063.5127442
CGTATGC335600.063.150644
TCTCGTA327450.063.0419841
TATGCCG337500.062.8101146
ATGCCGT340150.062.37803347
ATCTCGT335350.061.50584840
GATCTCG337500.061.07048839
TGCCGTC351000.060.37265448
CCGTCTT352350.060.1345850
GCCGTCT355350.059.6167649
CGTCTTC359400.059.01114751
GTATGCC359800.059.00490645
CGTCTGA377450.057.66995216
ACACGTC385800.056.82909813
ACGTCTG384550.056.63221415
AGTGATC375450.055.11098536
CACGTCT396700.055.0646314
GCTTGAA396100.053.6392359
GTGATCT387050.053.32373437
Produced by FastQC (version 0.11.5)