FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23727563
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC6543472.757750553649357TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT746900.064.12820442
TCGTATG768200.063.95817643
TCTCGTA748250.063.8512941
CGTATGC770150.063.82872844
AATCTCG748400.063.72608639
TATGCCG771200.063.63511346
ATGCCGT772700.063.51316547
ATCTCGT758100.062.9908140
CCGTCTT783450.062.54459450
CGTCTGA808900.062.50647416
ACACGTC813850.062.358213
TGCCGTC787100.062.32256348
ACGTCTG816200.061.99042515
GCCGTCT792800.061.81163449
GTATGCC796350.061.74448445
CGTCTTC794700.061.664151
CACGTCT824500.061.42952714
ATCGGAA847550.060.9824182
GATCGGA851800.060.5919271
GACCAAT809400.060.38621535
Produced by FastQC (version 0.11.5)