FastQCFastQC Report
Mon 23 Dec 2019
HTHH5BGXC_n01_GG054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHH5BGXC_n01_GG054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28304002
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC7975392.8177605414244953TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG888050.065.58465643
CTCGTAT882150.065.54516642
TATGCCG888900.065.5115246
CGTATGC892450.065.31541444
TATCTCG883950.065.2342739
ATGCCGT892250.065.2288747
TCTCGTA884550.065.20328541
CGATGTA894650.065.19369534
ATCTCGT893700.064.5759140
ACCGATG906100.064.5542332
CCGATGT904100.064.54333
CACCGAT907400.064.5199531
CCGTCTT907050.064.1548950
TGCCGTC909300.063.9969448
GCCGTCT912650.063.71126649
CGTCTTC917500.063.35585851
GTATGCC923300.063.1809945
TCACCGA930150.062.99501430
CGTCTGA945050.062.63324716
ACACGTC952100.062.3531213
Produced by FastQC (version 0.11.5)