FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37781228
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2558440.6771722719018026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGG43550.023.4649931
GATCCGC37600.022.8802551
GATCCCG37600.022.8802551
GATCACG53050.022.39881
GATCGTG63350.021.5330431
GATCTGC57750.019.8351361
GATCGCT44150.019.7011031
GATCCAC84400.019.4288371
GATCGCG25550.019.3469521
GATCATG96750.019.3068641
GATCAAC141000.019.2143571
GATCGTC76700.019.1484341
GATCCAG77300.018.815341
GATCCTG68350.018.427971
GATCACC62500.018.3276671
GATCCCC39450.017.9518591
GATCAGC74000.017.5990411
GATCGCC40450.017.2730461
GATCCGA76150.017.164571
GATCTCC75250.016.9277171
Produced by FastQC (version 0.11.5)