FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n02_DJ74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n02_DJ74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257157731
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG740100.024.6025711
GATCGCG335400.024.0416451
GATCCGC505850.023.5680871
GATCGTG886200.023.3079071
GATCAAC1847450.022.2041651
GATCCGG599250.021.353731
GATCCCG574500.021.2232571
GATCCAG1073700.020.2732221
GATCCTG906050.020.16461
GATCGTC1074200.020.0204961
GATCCAC1136450.019.5761151
GATCATG1367000.019.3959521
GATCCTC1162500.018.8840181
GATCCGA1058800.018.6556971
GATCAGC984750.018.5964971
GATCCGT781000.018.3191431
GATCCTT1818250.018.178221
GATCGCC537650.018.064531
GATCTGC841400.018.0375611
GATCGAC912250.017.9232271
Produced by FastQC (version 0.11.5)