FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n02_DJ73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n02_DJ73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342186330
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG439950.026.8497451
GATCCGC651550.026.385371
GATCACG961100.026.3413491
GATCGTG1160750.024.5000971
GATCCGG771200.023.9984281
GATCAAC2431050.023.52311
GATCCCG766150.023.4309831
GATCCAG1413900.022.03581
GATCCTG1210200.021.6222211
GATCCAC1471200.020.9224031
GATCATG1804650.020.7874641
GATCTGC1099700.020.7097841
GATCCGA1371700.020.7011181
GATCCTC1525450.020.6611441
GATCAGC1282850.020.6236571
GATCGTC1407950.020.5326181
GATCTCG1216850.020.17251
GATCCGT1015650.020.1078051
GATCGCC690500.019.6189561
GATCCTT2426400.019.3108271
Produced by FastQC (version 0.11.5)