FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n02_DJ72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n02_DJ72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131380933
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG172550.025.0039081
GATCACG377300.023.902671
GATCCGC258000.023.8315121
GATCCGG307700.022.7463781
GATCAAC943300.022.5363271
GATCGTG442000.022.3173221
GATCCCG296300.021.1519371
GATCCTG467200.020.6660691
GATCCAG555000.020.1192531
GATCCAC585550.019.7025171
GATCGTC554550.019.4329761
GATCATG705600.019.2594781
GATCAGC494200.019.0274281
GATCTGC422200.018.8509651
GATCCGA539200.018.7965431
GATCCTC596250.018.7512631
GATCGCC274050.018.6040251
GATCCGT390850.018.5516031
GATCGCA378400.017.8811661
GATCGCT313950.017.5109561
Produced by FastQC (version 0.11.5)