FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n02_DJ71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n02_DJ71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142627944
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC256200.027.2451821
GATCACG379800.027.1238521
GATCGCG171600.026.7489591
GATCGTG439200.024.9376541
GATCCCG298950.024.7637231
GATCAAC969600.024.2631591
GATCCTG480650.023.2517381
GATCCGG304550.023.1069971
GATCCAG546250.022.990731
GATCCAC589850.022.3546811
GATCTGC426150.021.7541351
GATCAGC497950.021.4801241
GATCGTC565450.021.0167961
GATCCGA529150.020.7703531
GATCATG709350.020.7590771
GATCGCC275550.020.6414781
GATCCGT395250.020.4493481
GATCCTC624300.020.375211
GATCGCT312850.020.2460821
GATCACC434250.020.0134031
Produced by FastQC (version 0.11.5)