FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n02_DJ70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n02_DJ70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134070664
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGG408750.025.702421
GATCCGC255200.025.5270021
GATCGCG181500.024.4257561
GATCACG371500.023.3296931
GATCCCG294200.021.6585391
GATCGTG430600.021.6505031
GATCAAC913950.020.5984751
GATCCAG621050.020.5581551
GATCCGT455350.019.8058681
GATCGCT395500.019.667321
GATCCAC606550.019.0754761
GATCTGC431600.018.5837781
GATCCGA502400.018.1969451
GATCAGC500050.018.0544051
GATCCTG502750.017.9574451
GATCCCC327200.017.6007961
GATCATG727800.017.4513871
GATCTCG499100.017.4223421
GATCCTT1282850.017.3197521
GATCGCA370650.016.883591
Produced by FastQC (version 0.11.5)